Drinking natural mineral water (microbiological) testing

8.3 Instruments and equipment 8.3.1 Burette: 25 mL. 8.3.2 Pipette: 50 mL, 25 mL, and 5 ml. 8.3.3 Conical flask: 150 mL. 8.3.4 Analytical balance; The sensitivity is 0.01 g. 8.4 Analysis Step 8.4.1 Take 50.0 mL of water sample (if the hardness is too high, a small amount of water sample can be taken and diluted with water to 50 ml. If the hardness is too low, use 100 mL instead), and place it in a 150 mL conical flask. 8.4.2 Add 1 mL~2 ml. buffer solution and 5 drops of chrome black T indicator, and immediately titrate with EDTA-2Na standard solution until the solution changes from purple red to unchanged sky blue. At the same time, perform a blank test and record the dosage. 8.4.3. If the water sample contains metal interfering ions that delay the titration endpoint or darken the color, another water sample can be taken, and 0.5 ml of hydroxylamine hydrochloride and 1 mL of sodium sulfide solution or 0.5 mL of potassium cyanide solution can be added before titration. 8.4.4: When the calcium and magnesium content in the water sample is high, the water sample should be pre acidified and heated to remove carbon dioxide to prevent the formation of carbonate precipitates after alkalization, which is difficult to convert during titration

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