Detection of genetically modified components in soybeans

Design specific primers based on the exogenous insertion fragment of DP-305423 and the plant genome sequence, use the lectin gene (118 bp) as the internal reference gene, screen the optimal primers, and optimize the reaction program and system. Finally, establish a specific PCR detection method for transgenic soybean DP-305423 transformant. The specificity, sensitivity, stability, and repeatability of this method were tested. The results indicate that this method can specifically detect DP 305423 soybean; The DP 305423 transgenic soybean and its receptor were mixed by mass ratio, and the sensitivity of detection reached 0.05%, which is about 20 initial template copies; Using samples with DP-305423 soybean DNA mass fractions of 1.00%, 0.10%, and 0.05% as templates, it has good stability, high repeatability, and a false negative rate of 0. The method designed in this experiment is suitable for the specific qualitative PCR detection of DP-305423 transgenic soybean

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