Detection of tetracycline, oxytetracycline, and aureomycin

Determination of Oxytetracycline, Tetracycline, and Chloramphenicol Residues in Livestock and Poultry Meat (High Performance Liquid Chromatography) 1 Scope This standard specifies the detection method for Oxytetracycline, Tetracycline, and Chloramphenicol Residues in Livestock and Poultry Meat. This standard is applicable to the determination of tetracycline and chlortetracycline residues in various livestock and poultry meat. The detection limit of this standard is 0.15 mg/kg for oxytetracycline, 0.20 mg/kg for tetracycline, and 0.65 mg/kg for chlortetracycline. The principle sample is extracted, filtered by a microporous filter membrane, and directly injected. It is separated by reverse phase chromatography, detected by a UV detector, and quantitatively compared with the standard. The peak sequence is oxytetracycline, tetracycline, and chlortetracycline. Standard addition quantification. 3 Reagents 3.1 Acetonitrile (Analytical Purity). 3.2 0.01 mol/L Sodium dihydrogen phosphate solution: Weigh 1.56 g (accurate to ± 0.01 g) of sodium dihydrogen phosphate (NaH, pH · 2HO) and dissolve it in distilled water, dilute to 100 ml, filter through a microporous filter membrane (0.45 pm), and set aside. 3.3 Oxytetracycline (OTC) standard solution: Weigh 0.010 0 g of Oxytetracycline (accurate to ± 0.000 1 g), dissolve it in 0.1 mol/L hydrochloric acid solution, and make a constant volume of 10.00 mL. This solution contains 1 mg of Oxytetracycline per milliliter. 3.4: Tetracycline (TC) standard solution: Weigh 0.0100 g of tetracycline (accurate to ± 0.001 g), dissolve it in 0.01 mol/L hydrochloric acid solution and make a constant volume of 10.00 mL. This solution contains 1 mg of tetracycline per milliliter. 3.5 Chloramphenicol (CTC) standard solution: Weigh 0.0100 g of tetracycline (accurate to ± 0.000 1 g), dissolve it in distilled water and make a constant volume of 10.00 mL. This solution contains 1 mg of tetracycline per milliliter. The above standards are converted to 1000 units/mg. 3.3-3.5 The solution should be stored below 4 ℃ and can be used for one week. 3.6 Mixed standard solution: Take 1.00 mL of 3.3 and 3.4 standard solutions respectively, take 2.00 mL of 3.5 standard solution, place in a 10 mL volumetric flask, and add distilled water to the mark. This solution contains 0.1 mg of oxytetracycline, 0.1 mg of tetracycline, and 0.2 mg of chlortetracycline per milliliter. It should be prepared immediately before use. 3.75% perchloric acid solution

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