Vodka (Odec detection

5.5.2.4.2 Drawing of Standard Curve: Take 2.0 mL of each series of standard solutions and place them in 25 mL stoppered colorimetric tubes. Add 2.0 mL of hydroxylamine hydrochloride solution (5.5.2.3.1) and 2.0 mL of sodium hydroxide solution (5.5.2.3.2) to each, shake the hook, and leave for 10 minutes. Then add 2.0 mL of hydrochloric acid solution (5.5.2.3.3) and shake well. Add 2.0 mL of iron chloride solution (5.5.2.3.4) and mix well again. Using a 1 cm colorimetric dish and adjusting the zero point with a zero tube, immediately measure the absorbance at a wavelength of 525 nm. Draw a standard curve. 5.5.2.4.3 Determination of sample solution: Take 2.0 mL of sample solution (5.5.2.4.1) into a 25 mL colorimetric tube with a stopper, and perform the remaining operations according to 5.5.2.4.2. Find the ethyl acetate content, i.e. the total ester content, on the standard curve. Alternatively, use a linear regression equation to calculate its content. The absolute difference between two independent measurement results obtained under repeatability conditions should not exceed 10% of the average value. 5.6 Methanol 5.6.1 has the same principle as 5.4.1.1. 5.6.2 The instrument is the same as 5.4.1.2. 5.6.3 Reagents and Solutions 5.6.3.140% Ethanol Solution: Prepare with ethanol (color harmonic purity) and water. 5.6.3.2 Methanol solution (2%): used as a standard sample. Take 2 mL of methanol (chromatographically pure) and dilute to 100 mL with a 40% ethanol solution. 5.6.3.3 n-butanol solution (2%): used as an internal standard. Take 2 mL of n-butanol (chromatographically pure) and bring to volume with a 40% ethanol solution to 100 mL. 5.6.4 Chromatographic conditions

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