（5） Biological testing

Baijia.net - a professional third-party testing and inspection platform that provides testing (V) biological testing and other related testing and ins

Food safety, as a major global public health issue related to the national economy and people's livelihood, has also received great attention. With the rapid development of the food industry, more technological means are needed to monitor the quality of the food production process and final products

From the statistical results below, it can be seen that among numerous food safety incidents, microbial contamination is the most important factor causing foodborne diseases. Therefore, as an important means of health management and safety indicator evaluation, establishing fast, accurate, and sensitive modern microbial detection methods plays a more crucial role in food safety monitoring

1. Disadvantages of Traditional Microbial Detection Methods

Traditional national standard detection methods are based on microbial culture. In order to explore fast and efficient microbial detection methods, we will first understand the problems of traditional national standard microbial detection methods:

1.1 Operation complexity

Conventional testing requires preparation of culture medium, sterilization, inverted plate, multi-level dilution, inoculation, cultivation, counting, and separation.

, Multiple operational steps such as identification. The testing operation is complex, and in order to obtain accurate test results, the technical requirements of the operators will be relatively high

1.2 Testing takes a long time

The most common method is to cultivate the total number of bacterial colonies for 48 hours, which is the least time-consuming traditional method.

. If positive bacteria are detected in items such as coliform bacteria, the entire testing time will be around 9 days

1.3 Large Consumable Materials

Based on the above operations, traditional detection methods require a large amount of culture medium, culture products, and other consumables.

. At the same time, the cultivation process requires a large number of incubators with different volumes and temperatures, and the energy consumption is also very high

2. Microbial rapid detection technology

Compared to traditional detection methods, rapid detection methods refer to the entire detection process from sample preparation to the issuance of detection results that can be completed in a short time, including simplified methods in sample preparation, experimental preparation, operation process, and automation

At present, the international consensus on "short time" mainly lies in three aspects:

1) The detection and analysis of physical and chemical indicators can be completed within 2 hours; 2) The application of on-site inspection can be completed within 30 minutes; 3) Compared with traditional methods, There are several methods for microbial rapid detection equipment that can shorten the time by 1/2 or 1/3.

2.1 Microbial Rapid Test Sheet Technology

Microbial Rapid Test Sheet is composed of two layers of thin film, the lower layer of polyethylene film is printed with a grid and covered with the culture medium required for bacterial growth, and the upper layer is polyethylene film. When using, simply peel off the upper layer of the film, inoculate the diluted solution of the sample to be tested into the lower layer of the culture medium, and place it in the incubator for cultivation and counting. At the same time, the indicator is preset in the culture medium to display a special color on the target colony, making it convenient for accurate interpretation. The product includes test pieces for total bacterial count, coliform bacteria, mold yeast, and various pathogenic bacteria

2.2 Immunological Detection Techniques

Immunological detection methods mainly include immune chromatography, enzyme immunoassay, immunofluorescence, and other types. The immune technologies used in food microbiology detection mainly include the following two types:

1) ATP luminescence detection technology.

. This detection method mainly relies on the biological characteristics of ATP to detect various microorganisms in food. The principle is to use fluorescein, fluorescein, and oxygen as substrates to undergo corresponding chemical reactions with microorganisms in food, thereby effectively converting chemical energy into light energy. This method does not require cultivation of food microorganisms and has high sensitivity. A common device is an ATP fluorescence detector, which can generally obtain test results in about 15 seconds

2) Latex agglutination reaction, the experimental phenomenon of which is relatively intuitive and can be directly observed with the naked eye. This technology utilizes the specificity of antigen antibodies to add artificially synthesized macromolecular latex particles to the reaction system, which can effectively and intuitively observe the aggregation reaction of particles. Based on the corresponding detection results, preventive measures are taken to reduce the probability of various harmful microorganisms in food. This method greatly shortens the detection time and also reduces the training time and requirements for operators

2.3 Molecular Biological Detection Technology

Among molecular biological detection methods, polymerase chain reaction (PCR) technology and gene chip technology are the most representative.

. PCR technology is a molecular biology technique used to amplify specific DNA fragments, which can be seen as in vitro DNA replication. The biggest feature of PCR is its ability to significantly amplify small amounts of DNA. The main advantages of this testing method are high specificity and fast detection speed

Gene chip technology is based on molecular biology and microelectronics technology. After fusing gene probes with the chip, the chip is scanned by a detection system to determine the presence of certain specific microorganisms. In theory, biochip technology can detect all potential pathogens and genetic indicators in one experiment. Therefore, in data processing and information extraction, the positioning of hybrid samples should be analyzed reasonably to avoid the impact of the chip on the automatic recognition results

Function of testing report:

1. Project bidding: Issue authoritative third-party CMA/CNAS qualification report

2. Online e-commerce platform entry: Quality inspection report recognized by major e-commerce platforms

3. Used as a sales report: issuing legally effective testing reports to make consumers more confident

4. Papers and research: Provide professional personalized testing needs

5. Judicial services: providing scientific, fair, and accurate testing data

6. Industrial problem diagnosis: Verify the troubleshooting and correction of industrial production problems

BAIJIAN testing process:

1. Telephone communication and confirmation of requirements

2. Recommend solutions and confirm quotations

3. Mail samples and arrange testing

4. Progress tracking and result feedback

5. Provide reports and after-sales service

6. If urgent or priority processing is required

Our Competitive Edge:

1. The testing industry is fully covered, meeting different testing needs

2. Fully cover the laboratory and allocate localized testing nearby

3. Engineers provide one-on-one services to make testing more accurate

4. Free initial testing, with no testing fees charged

5. Self service order delivery for free on-site sampling

6. Short cycle, low cost, and attentive service

7. Possess authoritative qualifications such as CMA, CNAS, CAL, etc

8. The testing report is authoritative and effective, and is generally used in China