Enzyme activity detection

Due to the highly specific catalytic activity of enzymes, their enzyme activity can be determined by measuring the corresponding substrate or product concentration changes, or by using a certain reaction product or reactant concentration change. Generally, electrochemical and photophysical methods are used, which utilize the absorbance of reactants or products and determine them using ultraviolet spectrophotometry or fluorescence method. If there is gas in the product or reactant during the enzyme reaction process, it can be measured using a manometer (Waals breathing apparatus). If acids are generated during the reaction process, electrochemical methods can be used. Substrates labeled with isotopes can be measured by radiochemical methods to determine changes in substrate concentration and calculate enzyme activity. Some enzymes with stable properties can also be detected by high-performance liquid chromatography. What are the items that require enzyme activity testing? Cosmetics, milk and dairy products (milk and milk powder, casein and casein powder, whey and whey protein powder, etc.), oil seeds (grains such as soybean products), feed, feed cellulose, industrial fats, feed phytic acid β- Baijian Group can provide you with enzyme activity testing services for various products such as milk and milk powder, casein and casein powder, feed, whey and whey protein powder, etc. Baijian is a scientific testing institution with a short research and development cycle, low costs, and complete experimental plans. The testing report issued is scientific, fair, and accurate, and supports global scanning to check authenticity, making it more convenient to use! Testing items: Enzyme activity testing. Enzyme activity testing scope: telomerase activity testing, protease activity testing, amylase activity testing, hyaluronidase activity testing, trypsin activity testing, alkaline phosphatase activity testing, pepsin activity testing, pectinase activity testing, polymerase activity testing, glycogen synthase activity testing, aldehyde dehydrogenase activity testing, thrombin activity testing, urease activity testing, Nitrogenase activity detection, lipase activity detection, etc. Standard for enzyme activity detection AS 2300.110-2008 Chemical and physical test methods in the dairy industry - Part 1.10: General methods and principles - Determination of phosphatase activity BS 4325-6-156 0190 2607 Method for analysis of oil seed residues - Part 6: Determination of urease activity in soybean products DB37/T 577-2005 Feed additives - Determination of 6-site phytase activity - Spectrometric method DIN EN ISO 11816-1-2006- Determination of alkaline phosphatase activity in milk and dairy products - Part 1 Part: Fluorescence method for milk and dairy beverages DIN EN ISO 30024-2009 Animal feed - Determination of inositol hexaphosphatase activity DIN ISO 23753-1-2006 Soil quality - Determination of soil dehydrogenase activity - Part 1: Triphenyltetrazolium chloride method (TTC) DIN ISO 23753-2-2006 Soil quality - Determination of soil dehydrogenase activity - Part 2: Tetrazolium chloride method (INT) GB/T 5521-2008 Grain and oil inspection - Cereals and their products α- Determination of amylase activity Colorimetric method GB/T 8622-2006 Determination of urease activity in soybean products for feed GB/T 18634-2009 Determination of phytase activity for feed GB/T 23881-2009 Filter paper method GB/T 30990-2014 Lysozyme activity detection method GB/T 32131-2015 Horseradish peroxidase activity detection method Colorimetric method GB/T 33409-2016 β- Detection method of galactosidase activity Spectrophotometry GB/T 34795-2017 Detection method of glutamine transaminase activity GB/T 34799-2017 Detection method of chitinase activity GB/T 35808-2018 Analysis method of forestry biomass raw materials Cellulase activity determination GB/T 36756-2018 General requirements for determination of tool enzyme activity GOST 13979.9-1969 Determination method of urease activity of Youbing (Deep-fried round and flat dough-cake) and oil cake GOST R 53360-2009 Detection method of phytase activity of enzyme preparation IDF 53-196 Determination of phosphatase activity in cheese stabilized by pasteurization (reference method) IDF 63-1971 Milk and milk powder, casein and casein powder Whey and whey powder: Determination of phosphatase activity ISO 13082-2011 Milk and dairy products - Determination of lipase activity in gastric lipase preparations ISO 23753-1-2005 Soil quality - Determination of soil dehydrogenase activity - Part 1: Triphenyltetrazolium chloride method ISO 30024-2009 Animal feed - Determination of inositol hexaphosphate activity ISO/TR 140-1008-2004 Milk and milk powder, casein and casein powder Detection of Phosphatase Activity in Whey and Whey Protein Powder ISO/TS 17193-2011 Determination of Milk Oxidase Activity Photometric Method ISO DIS 13082-2010 Determination of Lipase Activity in Pregastric Lipase Preparations of Milk and Dairy Products JIS K0601-1995 Method for Determination of Industrial Lipase Activity KS HISO 11816-1-2001 Fluorescence Method for Measurement of Alkaline Phosphatase Activity in Milk and Dairy Products Part 1: Milk and Dairy Beverages NF V03-942-1980 (R2001) Oilseed cake and soybean products - Determination of urease activity - NF EN ISO 11816-1-2006 Milk and milk products - Determination of alkaline phosphatase activity using fluorescence method - Part 1: Milk and milk beverages SN/T 0800.4-2015 Export of grain Feed inspection Part 4: Determination of urease activity T/SHRH 015-2018 Cosmetics Tyrosinase activity inhibition experimental method WS/T 66-1996 Whole blood cholinesterase activity spectrophotometric method hydroxylamine ferric chloride method WS/T 222-2002 Clinical enzyme activity concentration determination method General principles GB/T 5521-2008 Grain and oil inspection Cereals and their products α- Determination of amylase activity Colorimetric method GB/T 8622-2006 Determination of urease activity in soybean products for feed GB/T 18634-2009 Determination of phytase activity for feed GB/T 23881-2009 Filter paper method GB/T 30990-2014 Lysozyme activity detection method GB/T 32131-2015 Horseradish peroxidase activity detection method Colorimetric method GB/T 33409-2016 β- Method for detecting galactosidase activity - Spectrometric method GB/T 34795-2017 Method for detecting glutamine transaminase activity GB/T 34799-2017 Method for detecting chitinase activity GB/T 35808-2018 Method for analyzing forestry biomass raw materials - Determination of cellulase activity - Enzyme activity detection report - What are the purposes of this report? 1. Sales use. (Issuing third-party testing reports allows customers to trust their product quality more, which can have certain advantages in sales and increase success rates!) 2. Quality control use. (Improve product quality through testing report data and internal quality control by manufacturers) 3. Use of data from university and scientific research papers. 4. Import and export use (China National Institute of Chemical Industry's testing report supports global scanning to distinguish authenticity) 5. Research and development of new products for use. The above article is a partial list. For more testing needs and details, please consult the online consultant of the free consulting agency: 15201733840 (phone and WeChat). We conduct testing on the top 100 inspection websites - issuing authoritative testing reports has legal effect.

Function of testing report:

1. Project bidding: Issue authoritative third-party CMA/CNAS qualification report

2. Online e-commerce platform entry: Quality inspection report recognized by major e-commerce platforms

3. Used as a sales report: issuing legally effective testing reports to make consumers more confident

4. Papers and research: Provide professional personalized testing needs

5. Judicial services: providing scientific, fair, and accurate testing data

6. Industrial problem diagnosis: Verify the troubleshooting and correction of industrial production problems

BAIJIAN testing process:

1. Telephone communication and confirmation of requirements

2. Recommend solutions and confirm quotations

3. Mail samples and arrange testing

4. Progress tracking and result feedback

5. Provide reports and after-sales service

6. If urgent or priority processing is required

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1. The testing industry is fully covered, meeting different testing needs

2. Fully cover the laboratory and allocate localized testing nearby

3. Engineers provide one-on-one services to make testing more accurate

4. Free initial testing, with no testing fees charged

5. Self service order delivery for free on-site sampling

6. Short cycle, low cost, and attentive service

7. Possess authoritative qualifications such as CMA, CNAS, CAL, etc

8. The testing report is authoritative and effective, and is generally used in China