Microbial testing of food

This standard specifies the inspection method for Listeria monocytogenes in food. The first method of this standard is applicable to the qualitative inspection of Listeria monocytogenes in food; The second method is applicable to the counting of Listeria monocytogenes in foods with high levels of Listeria monocytogenes; The third method is applicable to the counting of Listeria monocytogenes in foods with low levels of Listeria monocytogenes (< 100 CFU/g) and high levels of miscellaneous bacteria, especially in milk, water, and foods containing particulate matter that interferes with colony counting. 2. Equipment and materials: In addition to conventional sterilization and cultivation equipment in the microbiological laboratory, other equipment and materials are as follows: 2.1 Refrigerator: 2 ℃~5 ℃. 2.2 Constant temperature incubator: 30 ℃ ± 1 ℃. 36 ℃ ± 1 ℃. 2.3 Homogenizer. 2.4 Microscope: 10 x~100 x.2.5 electronic balance; Sensitivity 0.1 g. 2.6 Conical flask: 100 mL, 500 mL. 2.7 Sterile pipette: 1 mL (with a 0.01 mL scale), 10 mL (with a 0.1 mL scale) or micropipette and pipette. 2.8 Sterile plate: diameter 90 mm. 2.9 Sterile test tube: 16 mm × 160 mm. 2.10 Centrifugal tube: 30 mmX100 mm. 2.11 Sterile syringe: 1 ml. 2.12 Listeria monocytogenes ATCC 19111 or CMCC 54004, or other equivalent standard strains. 2.13 Listeria innocuia ATCC 33090, or other equivalent standard strain. 2.14 Listeria ivanovii ATCC 19119, or other equivalent standard strain. 2.15 Listeria seeligeri ATCC 35967, or other equivalent standard strain. 2.16 Staphylococcus aureus ATCC 25923 or other strains β- Hemolytic Staphylococcus aureus, or other equivalent standard strains. 2.17 Rhodococcus equi ATCC 6939 or NCTC 1621, or other equivalent standard strains. 2.18 White mice: ICR weighing 18 g~22 g. 2.19 Fully automated microbial biochemical identification system

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