Amino acid nitrogen detection

Amino acid nitrogen refers to soil nitrogen that exists in soil proteins and peptide compounds and can release various amino acids after degradation. It is the most abundant compound known in soil nitrogen, and over 30 amino acids have been identified in the soil. The dominant amino acids are usually found in microbial cell walls, such as glutamic acid, glycine, alanine, lysine, and aspartic acid. Amino acid nitrogen in soil mainly exists in the following ways: ① free amino acids, with very low content, mainly present in soil solutions and soil micropores; ② Existing in the binding state of amino acids, peptides, or proteins with clay minerals, bound to the surface or lattice of clay particles; ③ Existing in the form of amino acids, peptides, or protein organic colloids, bound by hydrogen bonds, peptide bonds, or covalent bonds; ④ Viscopeptide-containing substances derived from microbial cells; ⑤ Glue peptide substances that bind to sugars (such as microbial cell walls); ⑥ It exists in the form of phosphorus wall (acid) substance, which is a linear polymer of polyols or a phosphoglycerol containing ester bonds linked to alanine

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