Food (reducing sugar) testing

GB 5009.7-2016 "National Food Safety Standard - Determination of Reducing Sugar in Foods" specifies the method for determining reducing sugar, with the more classic being the "direct titration method". The main steps include sample preparation, calibration of alkaline copper tartrate solution, prediction of sample solution, determination of sample solution, and calculation of analytical results. The sample solution was predicted first and then determined, and a total of two titrations were performed

The prediction of sample solution is to use a bottle mouth distributor to transfer 5.0mL of alkaline copper tartrate solution A and 5.0mL of alkaline copper tartrate solution B into a 150mL conical flask, add 10mL of water, add 2-4 glass beads, and control the heating to boiling within 2 minutes to maintain boiling. Add the sample solution dropwise from the Opus electronic titrator. First, perform a rapid titration using the titration mode. When the color of the solution becomes lighter, titrate at a rate of half a drop per second using the half drop mode until the blue color of the solution just fades away as the endpoint. Record the value on the display screen as the consumed volume of the sample solution in V

Sample solution determination involves using a bottle mouth dispenser to transfer 5.0mL of alkaline copper tartrate solution A and 5.0mL of alkaline copper tartrate solution B into a 150mL conical flask, adding 10mL of water and 2-4 glass beads. The pre titration function of the Opus electronic titrator is used to set the volume to V-1mL and press the titration button to complete the addition. The solution is heated to boiling within 2 minutes, and then titrated in a half drop mode at a rate of half a drop/second until the blue color of the solution just fades away as the endpoint, Record the value on the display screen as the consumed volume of the sample solution. Operate three parts in parallel using the same method to obtain the average consumed volume

The bottle mouth dispenser and Opus electronic titrator from Herschmann, Germany, can replace conventional measuring cylinders, pipettes, and burets, making it more convenient and safe to use direct titration to determine reducing sugars in food

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