Detection of Lycium barbarum polysaccharides

Method 1: Detection of Lycium barbarum polysaccharides: The crude polysaccharides are hydrolyzed into monosaccharides under the action of sulfuric acid, and rapidly dehydrated to form aldehyde derivatives. They are then condensed with phenol to form colored compounds. The content of the crude polysaccharides in the sample is determined by spectrophotometry. 2. Main equipment and instrument reagents: Standard solution of Lycium barbarum polysaccharides: Accurately weigh 0.1000g of Lycium barbarum polysaccharides, dried at 105 ℃ and constant weight, and place it in a 100mL volumetric flask. Dissolve and dilute it with distilled water to the mark, with a concentration of 1.0mg/mL. Dilute it to a standard working solution with a concentration of 0.1mg/mLd. 5% phenol solution: Take 100g of phenol and distill it in a sand bath. Collect 156, 190, 2607 ℃ -182 ℃ for distillation, weigh 5g of this fraction, dissolve it in water, and bring it to a constant volume in a 100mL brown volumetric flask for later use (ready to use). 3. Determination Step 3.1, Selection of Maximum Absorption Wavelength: Precisely absorb 0.04mg/mL of anhydrous goji berry polysaccharides and dissolve 1.0mL. Place it in a 10mL stoppered test tube, add distilled water to a volume of 2.0mL, and then add 1.0mL of phenol test solution. Shake well and quickly add 5.0mL of concentrated sulfuric acid. Shake well and let it stand for 5 minutes. Heat it in a boiling water bath for 15 minutes, remove it, and cool it to room temperature. Use distilled water instead of sugar solution to prepare a blank using the addition method. Measure the absorbance at 470-510nm using a 721 spectrophotometer, with a maximum absorption wavelength of 490nm. 3.2. Drawing of Standard Curve: Precision absorption concentration of 0.1mg/mL goji berry polysaccharide standard. Standard working solutions of 0, 0.20, 0.30, 0.40, 0.60, and 0.80mL were placed in 10mL stoppered test tubes, each with distilled water added to a volume of 2.0mL. Then, 1.0mL of phenol test solution was added, shaken well, and 5.0 mL of concentrated sulfuric acid was quickly added. After shaking well, it was placed for 5 minutes, heated in a boiling water bath for 15 minutes, and cooled to room temperature after removal. Prepare a blank using distilled water instead of sugar solution as described above. Using a 721 spectrophotometer and a 1cm cuvette, measure the absorbance at 490nm and draw the glucose concentration absorbance working curve. The concentration range between 0.010 and 0.04mg/mL is correlated with absorbance. The regression equation Y=7.5210-3X+1.1310-3, Y=0.9997 (n=6). 3.3. Content Determination 3.31. Extraction and Refinement of Polysaccharides: Take 300mL, extract multiple times with chloroform to remove protein, add 1% activated carbon for decolorization, filter, and add 95% ethanol to the filtrate to achieve an alcohol content of 80%. Let it stand overnight. Filter, wash the residue with anhydrous ethanol, acetone, and ether multiple times, and vacuum dry to obtain crude polysaccharides. 3.32. Preparation of test solution: Weigh 0.2000g of crude polysaccharide powder accurately, place it in a 50mL volumetric flask, fix the volume, and shake well to obtain the test solution. 3.33. Precision of Sample Content Determination: Take 2.0mL of the test solution and measure the absorbance according to the method under "Standard Curve Drawing". 4. Calculate the polysaccharide content (%)=(CD)/V100 in the formula: C - glucose concentration of the test solution (mg/mL); D - Dilution factor of test solution; V - Volume of test solution (mL). 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A: The company stipulates that all testing services under its umbrella are available for free consultation before and after sales. Once a problem is raised by a customer, a solution is provided within 2 days and implemented within 7-10 days Q: How are you going to sign your contract? A: After you have determined the items to be tested, our sales team will promptly prepare the relevant contract, and then fax, send scanned copies or express delivery according to your needs. The contract can only take effect after both parties sign it Q: What is your payment method? A: Within the urban area of Shanghai, payment can be made in person after signing a contract. Customers from other locations adopt the principle of payment before delivery. If customers have any doubts, they can inspect the laboratory before placing an order or conduct factory inspections after placing an order Q: Do you package the test report? 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Users can directly find qualified institutions to place orders at Baijian Mall, or consult customer service to recommend direct orders from nearby laboratories Our vision is to build a leading comprehensive service platform for comprehensive quality assurance of the Internet in China through the Baijian brand Baijian platform. Baijian is committed to utilizing innovative internet technology, working with numerous authoritative large, medium, and small inspection, testing, and certification service institutions, surpassing traditional quality control methods, and always providing professional, accurate, fast, and enthusiastic comprehensive quality assurance services to ensure customers win the market. 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