Microbial testing in food packaging materials

This standard specifies the inspection method for Cronobacter in food. This standard is applicable to the inspection of Cronobacterium in infant formula food, milk, dairy products, and their raw materials. 2. Equipment and materials: In addition to conventional sterilization and cultivation equipment in the microbiological laboratory, other equipment and materials are as follows: 2.1 Constant temperature incubator: 25 C ± 1 C, 36 C ± 1 C.44 C ± 0.5 C.2.2 Refrigerator: 2 C~5 C.2.3 Constant temperature water bath: 44 C ± 0.5 C.2.4 Balance: Sensitivity 0.1 go2.5 Homogenizer. 2.6 Oscillator. 2.7 Sterile pipette: 1 mL (with a 0.01 ml scale), 10 mL (with a 0.1 mI scale) or micropipette and suction head. 2.8 Sterile conical flask: capacity 100 mL, 200 mL, 2000 ml. 2.9 Sterile culture dish: diameter 90 mm.2.10 pH meter or pH colorimetric tube or precision pH test paper. 2.11 Fully automatic microbial biochemical identification system. 3 Culture media and reagents 3.1 Buffer peptone water. BPW: see A.1.3.2 Modified lauryl sulfate tryptophase broth vancomycin medium. MLST-Vm: see A.2. 3.3 Coloring medium for Enterobacter sakazakii. 3.4 Tryptase soy agar (TSA): see A.3 3.5 Biochemical Identification Kit. 3.6 Oxidase reagent: see A.4.3.7 1L lysine decarboxylase medium: see A.5 3.8 L ornithine decarboxylase medium: see A.6.3.9 1-arginine dihydrolase medium: see A.7. 3.10 Sugar fermentation medium: see A.8. 3.11 Simmons citrate medium: see A.9

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