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This standard specifies the refractometer method for the determination of soluble solid content in fruits and vegetables. This standard is applicable to the determination of soluble solid content in fruits and vegetables. The following normative references are essential for the application of this document. For dated references, only the dated version applies to this document. For undated references, the latest version (including all modification orders) applies to this document. GB/T 8855 Sampling Method for Fresh Fruits and Vegetables - Principle 3: Use a refractometer to determine the refractive index of the sample solution. Read the soluble solid content of the sample solution from a display or graduated scale, expressed as a mass percentage of sucrose. 4 instruments and equipment 4.1 Refractometer: Brix scale is 0.1%. 4.2 High speed tissue crusher; Speed I0 o00 r/min~12000 r/min. 4.3 Balance: Sensitivity 0.01g.5 Sampling shall be carried out in accordance with the provisions of GB/T 8855. 6. Determination Step 6.1: Preparation of Fruits and Vegetables. Wash and wipe dry. Cut the edible portion into small pieces, mix well, and weigh an appropriate amount of sample (high moisture content sample is generally weighed at 250 g, while low moisture content sample is generally weighed at 125 g, with an appropriate amount of distilled water added). Place the sample in a high-speed tissue crusher and crush it. Use two layers of mirror paper or four layers of gauze to extrude the homogenate juice for measurement. 6.2 Instrument calibration at 20 ℃. Calibrate the refractometer with distilled water - adjust the reading of soluble solids content to 0. When the ambient temperature is not at 20 ℃, calibrate according to the calibration values in Appendix A. 6.3 Sample solution determination: Maintain a stable measurement temperature with a variation not exceeding ± 0.5 ℃. Wipe the surface of the prism with a soft velvet cloth. Add 2-3 drops of the sample solution to be tested, so that the sample solution is evenly distributed on the entire prism surface. Align the light source (non digital refractometer should rotate the achromatic adjustment knob to divide the field of view into two parts: light and dark. Then rotate the prism knob to make the light and dark boundary line fit at the intersection of the objective lens), and record the refractometer reading. A refractometer without automatic temperature compensation function to record and measure temperature. Wipe the surface of the prism clean with distilled water and a soft cloth. Note: During measurement, strong light interference should be avoided
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