Testing of milk and dairy products/food parameters

This standard specifies the determination method for L-hydroxyproline in raw milk. This standard is applicable to the determination of L-hydroxyproline in raw milk. The detection limit of this standard is 30 mg/L. The following normative references are essential for the application of this document. Only the dated version of the referenced documents in Er'en is applicable to this document. Any undated referenced documents are the latest version (Including the modification of the stone, this document GB/T 6682 is used to analyze the specifications and experimental methods of Sichuan water in the laboratory. The sample is hydrolyzed by acid and free. 1. It is oxidized by chloramine II on the elbow and converted into a vaporized substance containing a pyrrole ring. Amine benzaldehyde reacts to generate A complex 1nr version n, and converted into a compound containing a pyrrole ring. The product is related to dimethyl 4 reagents and materials. Unless otherwise specified, only reagents confirmed to be analytical pure are used in the analysis, and water is GB/T 66) Secondary water as specified in 82. 4.1 Hydrochloric acid (Hci. p. o g mlD), premium grade pure. 4.2 Hydrochloric acid solution< 0. D2 mot/1, take 1.7mL of hydrochloric acid (41) to a constant volume with water, shake to 4.3 buffer solution: Take 54.0g of citric acid (C1OHgoy, 2e. 3) G of hydrogen peroxide weabFy and 146.1 g of sodium acetate (NaO-C, Hs) residue were added to water, followed by adding 200 ml of water and 300 ml of zinc propylene MCOioH from gold zero to 16, and mixed. 4.4: Chloramine T solution, A amine T&, H1. CNlyin9. S). Dissolve in -10 ml of water, add 10 mL of n-propanol and 80 mL of Aichong solution in sequence (. 3> Shen: currently used 4.5 color reagent: weigh 0. b p-methylbenzaldehyde cHI NO3 and use 35 ml, as high as HelO.), slowly add 65 mL of isopropanol (CHZO) and mix well. Currently used 4.6 sodium hydroxide solution (1 o mol/): accurately weigh 400g of sodium argon oxide, dissolve and cool to a constant volume of 100 mL. 4.7 Sodium Hydroxide Solution (Accurately weigh 4.0g of sodium hydroxide at 1 mol/lq, dissolve in water and bring to 100 mL. 4.8 L-Hydroxyproline Standard Stock Solution (500nmg/LD): Accurately weigh 50.0 mg of L-Hydroxyproline Standard (CG. H, NO, CAS: 51-35-4, purity ≥ 99.0%), dissolve in 0.02 mol/L hydrochloric acid solution (4.2), bring to 100 mL. Store in a refrigerator at 4 ℃, with a validity period of 6 months. 4.9 L. hydroxyproline standard working solution (5.00 mg/L): Accurately pipette 1.00 ml of the standard stock solution (4.8) into a 100 ml volumetric flask, and bring to volume with 0.02 mol/L hydrochloric acid solution (4.2) to the mark. It is now ready for use

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