Food and health food (toxicology and function) testing

This standard specifies the basic technical requirements for the sex associated recessive lethal test of fruit flies. This standard is applicable to evaluating the genotoxic effects of the test substance. 2 Terminology and Definitions 2.1 A change that occurs in a lethal mutant genome. When expressed, it causes carrier death. 2.2 Recessive mutation is a change in the genome that is expressed only under homozygous or hemizygous conditions. 2.3 Genes associated with sex exist on the sex chromosome (X or Y). This only refers to genes located on the X chromosome. 3. Objective and principle of the experiment: Recessive genes have cross genetic characteristics in sexual inheritance, that is, the x chromosome of male flies is transmitted to the F generation male flies, and then to the F generation male flies through the F generation female flies. The recessive genes located on the X chromosome are heterozygous and cannot be expressed in the F generation male flies, but can be expressed in the semi homozygous F generation male flies. Based on this, the trait of eye color is determined by genes on the X chromosome and is associated with the inheritance of the X chromosome as a marker for observing gene mutations on the X chromosome. Therefore, wild-type male flies (red round eyed, normal flies) were infected and mated with Base (Muller 5) eagle flies (light apricot rod eyed, with one inversion on each X chromosome to prevent the F generation from exchanging the treated paternal and maternal X chromosomes). For example, after male flies were treated with the test substance, If a gene on the X chromosome undergoes recessive death, it can be expressed in the F generation of male flies based on the above two genetic rules, and the results of the experiment can be judged by using eye color traits as markers. According to Mendelian classification reaction, four different phenotypes of Fx generation were generated, and when there was a recessive death, there were no red round eyed male flies in the F generation. 4 Instruments and reagents 4.1 Instruments Electric heating constant temperature drying oven, biochemical incubator, three-dimensional anatomical microscope, magnifying glass, air conditioner, anesthesia bottle, fruit fly culture tube, test tube tray and rack. White porcelain board, sponge pad, brush, sponge plug Wash and disinfect the fruit fly feeding utensils at 120 ° C for 2 hours before use. 4.2 Reagents: Ether, 75% ethanol, acetone, Tween

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