Food and health food (nutritional composition) testing

This standard specifies the determination method for vitamin B in food. The first method of this standard is high-performance liquid chromatography, and the second method is fluorescence spectrophotometry, which is suitable for the determination of vitamin B in various foods. 2. The sample was hydrolyzed at constant temperature in a dilute hydrochloric acid environment and adjusted to pH between 6.0 and 6.5. It was hydrolyzed with papain and peak amylase, and filtered at constant volume. The filtrate was separated by a reverse phase chromatography column, detected by a high-performance liquid chromatography fluorescence detector, and quantified by an external standard method. Unless otherwise specified, all reagents and materials used in this method are analytical pure, and the water is the first grade water specified in GB/T 6682. 3.1 Reagent 3.1.1 Hydrochloric acid (HCD. 3.1.2 Glacial acetic acid (CH, COOH. 3.1.3 Sodium hydroxide (NaOH)). 3.1.4 Sodium acetate trihydrate (CH, COONa 3H, O.3.1.5 methanol (CH, OH); Chromatographic purity. 3.1.6 Papain; Activity unit ≥ 10 U/mg. 3.1.7 Peak amylase: Activity unit ≥ 100 U/mg, or equivalent in performance. 3.2 Reagent Preparation 3.2.1- Hydrochloric Acid Solution (0.1 mol/L): pipette 9 mL of hydrochloric acid, dilute with water and bring to volume 1000 ml. 3.2.2 Hydrochloric Acid Solution (1+1); Measure 100 mL of hydrochloric acid, slowly pour it into 100 mL of water, and mix well. 3.2.3 Sodium hydroxide solution (1 mol/L); Accurately weigh 4g of sodium hydroxide, dissolve in 90 ml. of water, cool, and bring to a constant volume of 100 mL. 3.2.4: Sodium acetate solution (0.1 mol/L): Accurately weigh 13.60 g of sodium acetate trihydrate, dissolve in 900 ml of water, and bring to volume with water to 1000 mL. 3.2.5 Sodium acetate solution (0.05 mol/L): Accurately weigh 6.80 g of sodium acetate trihydrate, dissolve in 900 mL of water, adjust the pH to 4.0-5.0 with glacial acetic acid, and bring to a constant volume of 1000 mL with water. 3.2.6 Mixed enzyme solution; Accurately weigh 2.345 g of papain and 1.175 g of peak amylase, dissolve them in water and bring to a constant volume of 50 mL. Prepare before use. 1

Function of testing report:

1. Project bidding: Issue authoritative third-party CMA/CNAS qualification report

2. Online e-commerce platform entry: Quality inspection report recognized by major e-commerce platforms

3. Used as a sales report: issuing legally effective testing reports to make consumers more confident

4. Papers and research: Provide professional personalized testing needs

5. Judicial services: providing scientific, fair, and accurate testing data

6. Industrial problem diagnosis: Verify the troubleshooting and correction of industrial production problems

BAIJIAN testing process:

1. Telephone communication and confirmation of requirements

2. Recommend solutions and confirm quotations

3. Mail samples and arrange testing

4. Progress tracking and result feedback

5. Provide reports and after-sales service

6. If urgent or priority processing is required

Our Competitive Edge:

1. The testing industry is fully covered, meeting different testing needs

2. Fully cover the laboratory and allocate localized testing nearby

3. Engineers provide one-on-one services to make testing more accurate

4. Free initial testing, with no testing fees charged

5. Self service order delivery for free on-site sampling

6. Short cycle, low cost, and attentive service

7. Possess authoritative qualifications such as CMA, CNAS, CAL, etc

8. The testing report is authoritative and effective, and is generally used in China