Lactic acid bacteria beverage/other physical and chemical tests

This standard specifies the qualitative determination method for urease in lactic acid bacteria beverages. This standard is applicable to the qualitative determination of urease in lactic acid bacteria beverages. Reagent 2.10.1% phenol red ethanol solution. 2.20.06 mol/L sodium dihydrogen phosphate: 4.689 NaHPO· 2H, О Dissolve in 500 ml. of water. 2.30.06 mol/L disodium hydrogen phosphate: 21.51 g Na: HPO· Dissolve 2H, O in 1000 mL of water. 2.410% urea solution. Step 3: Add 390 mL of 0.06 mol/L sodium dihydrogen phosphate buffer and 610 mL of 0 Mix 06 mol/L disodium hydrogen phosphate buffer solution. Take two test tubes, add 1 mL of each sample, and add 1 mL of mixed buffer solution. Add 2 mL of 10% urea solution to the sample, and 2 mL of distilled water to the control tube. Shake well and place in a 30 ℃ water bath for 10 minutes. Add 1-2 drops of phenol red indicator to the sample tube and the control tube, and compare the colors of the two tubes after shaking well. The control tube is yellow or orange yellow; The sample containing saponins appears bright red

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