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This standard specifies the method for determining the chlorophyll content in imported and exported animal and plant fats. This standard is applicable to the inspection of chlorophyll content in imported and exported Mao Cai seed oil. 2. The provisions contained in the following standards constitute the provisions of this standard by reference in this standard. At the time of publication of this standard, all versions shown are valid. All standards will be revised, and parties using this standard should explore the possibility of using the latest versions of the following standards. SN/T 0798-1999 Terminology for Inspection and Inspection of Import and Export Grain, Oil, and Feed SN/T 0799-1999 General Rules for Inspection and Inspection of Import and Export Grain, Oil, and Feed SN/T 0800.1-1999 Sampling and Sample Preparation Methods for Inspection of Import and Export Grain, Oil, and Feed 3. The following definitions are used in this standard. The chlorophyll determined by this method is the total content of chlorophyll and demagnesified chlorophyll in Maocai seed oil, measured by spectrophotometry and calculated as chlorophyll a. The sampling and sample preparation standards shall be carried out in accordance with SN/T 0800.1. 5 Test Method 5.1 Reagent Carbon tetrachloride: Chromatographic purity. 5.2 Instrument Shimadzu UV 260 spectrophotometer, or other equivalent instrument with dual beam scanning function. 1 cm -5 cm color comparison slots with different optical paths. 5.3 Operating Steps: Turn on the instrument switch, after the instrument self checks, select a wavelength range of 620 nm to 720 nm, adjust the oil temperature to 25 ℃± 2 ℃, use an appropriate optical path colorimetric groove, carefully pour the oil sample into the colorimetric groove, be careful not to cause bubbles in the oil sample, use carbon tetrachloride as a reference, and perform absorbance scanning within the range of 620 nm to 720 nm, so that the peak absorbance falls between 0.3-0.8, and record the absorbance of peaks and valleys, Refer to Appendix A
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